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Molecular cloning is the laboratory process used to create recombinant DNA.It is one of two most widely used methods, along with polymerase chain reaction (PCR used to direct the replication of any specific DNA sequence chosen by the experimentalist.
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The nucleotides are joined to one another in a chain by covalent bonds between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone.Great care, however, must be taken to prevent contamination with other biological materials during the identifying, collecting, and preserving of a sample.Kriaucionis S, Heintz N (May 2009).
There are two fundamental differences between the methods.
Recombinant DNA technology - Genomics: The genetic analysis of entire genomes is called genomics.
Such a broadscale analysis has been made possible by the development of recombinant DNA technology.
In humans, knowledge of the entire genome sequence has facilitated searching for genes that produce hereditary diseases.
It is also capable of revealing a set of proteinsproduced at specific.
Recombinant DNA technology - Gene therapy: Gene therapy is the introduction of a normal gene into an individuals genome in order to repair a mutation that causes a genetic disease.
When a normal gene is inserted into a mutant nucleus, it most likely will integrate into a chromosomal site different from the defective allele; although this may repair the mutation, a new mutation may result.